The Comparison of Entamoeba gingivalis Presence in Healthy and Periodontitis Patients by Using Direct Examination and PCR Methods

Rahdar*, Mahmoud; Abolfazli-Karizi, Salman; Pedram, Hootan

doi:10.5812/jjhs.86066

The Comparison of Entamoeba gingivalis Presence in Healthy and Periodontitis Patients by Using Direct Examination and PCR Methods

Document Type : Research Article

Authors

Mahmoud Rahdar* ¹

Salman Abolfazli-Karizi ²

Hootan Pedram ³

¹ Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

² Periodantics Department, Dentistary Faculty, Jundishapur University of Medical Sciences, Ahvaz, Iran

³ Dentistry Faculty, Jundishapur University of Medical Sciences, Ahvaz, Iran

10.5812/jjhs.86066

Abstract

Background:
The most frequent protozoan in the buccal cavity is Entamoeba gingivalis and more than 50% of the world population are infected with it. The exact role of E. gingivalis in periodontal diseases remains uncertain.
Objectives:
This cross-sectional descriptive study was conducted to compare the presence of E. gingivalis in healthy individuals and patients.
Methods:
Twenty-five patients with periodontitis and 25 healthy controls were selected and their saliva and plaque samples were examined by microscopy and PCR methods.
Results:
Only 3 out of the 25 patient samples were positive in microscopy examination, and four patient and two control group samples were positive in the PCR technique. There were no significant differences between the two groups (P > 0.05) using Chi-square test.
Conclusions:
: In the current study, E. gingivalis was not found to cause periodontal diseases. Studies with larger sample sizes are required to define the exact pathogenic role of E. gingivalis.
Keywords:Amoebiasis,Periodontitis,Oral Cavity,PCR

Keywords

Amoebiasis

Periodontitis

Oral Cavity

PCR