Document Type : Research Article
Authors
1
Department of English Language, Infectious Diseases Centers, School of Medicine, Yazd Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2
Department of Medical Parasitology and Mycology, Infectious Diseases Center, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
3
Department of Pharmacognosy, School of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
4
Community Medicine, Monitoring of Health Research Center, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
5
Department of Microbiology, Immunology and Parasitology, School of Medicine, Qom University of Medical Sciences, Qom, Iran
6
Department of Basic Medical Sciences, Maragheh University of Medical Sciences, Maragheh, Iran
7
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
10.5812/jjhs-148933
Abstract
Background:
Cutaneous Leishmaniasis (CL) is a widespread parasitic disease that causes skin lesions and ulcers. Although the World Health Organization recommends Glucantime as a treatment for CL, this remedy presents unexpected side effects and high costs. This study investigates the in vitro antileishmanial effect of Scrophulariabavanatia hydroalcoholic extract on Leishmaniatropica promastigotes.
Objectives:
This study aims to evaluate the in vitro antileishmanial effects of a local Scrophulariabavanatia hydroalcoholic extract on Leishmania (L.) tropica [MHOM/NADIM3] promastigotes.
Methods:
Various concentrations (0.2, 1, 5, 25, and 125 mg/mL) of Scrophulariabavanatia methanol extract were prepared and added to cultures containing the parasite. The amastigote form of the L. tropica strain [MHOM/IR/NADIM3] was isolated from mouse spleens and transformed into promastigotes in Novy-Nicolle-Mac Neal medium. The antileishmanial activity of Scrophulariabavanatia extract was then compared to Glucantime using cell proliferation ELISA.
Results:
The LC50 values in the stationary stage for both Scrophulariabavanatia extract and Glucantime (mg/mL) were greater than in the dynamic stage. The extract at concentrations of 0.2, 1, 5, 25, and 125 mg/mL did not show significant differences after 24 and 48 hours (P > 0.05) compared to negative controls but did show a significant effect after 72 hours (P = 0.000). The extract demonstrated increased effectiveness with higher concentrations and longer exposure times.
Conclusions:
Scrophulariabavanatia extract inhibits the survival and growth of Leishmania promastigotes. Future studies should further explore its effects on in vivo survival of the parasite.
Keywords:Leishmania tropica/Antileishmanial/Scrophularia bavanatia/Promastigotes/in vitro
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