1
Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, IR Iran
2
Graduated from Department of Food Hygiene, faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, IR Iran
10.17795/jjhs-31195
Abstract
Background: Escherichia coli O157:H7 (EHEC) has been traditionally associated with foodborne infections from consumption of foods with animal origin such as ground beef and burgers. Objectives: The objective of this study was to investigate the contamination of fresh ground beef with non-sorbitol fermenting Escherichia coli and presence of virulence genes in isolates obtained from butchers located in Ahvaz, Iran. Materials and Methods: A total of 200 fresh ground beef were sampled during a six-month period. All samples were enriched in Tryptic Soy Broth (TSB) with novobiocin and plating on Cefixime Telluride-Sorbitol MacConkey (CT-SMAC). The suspected colonies were subjected to Polymerase Chain Reaction (PCR) analysis to identify virulence genes containing rfbE O157, flic H7, stx1 and stx2 genes. Results: Overall, 1.5% of ground beef samples were contaminated with the O157 E. coli strain meanwhile 1% of samples contained the O157:H7 strain and 0.5% of samples had the O157:H7 with virulent stx1 and stx2 genes. Conclusions: The observed results indicated the necessity of good care in abattoir, butcheries and during food distribution, mainly ground beef. Also, a zero tolerance policy could be useful to control E. coli O157 in meat products nationwide.
Maktabi, S., Zarei, M. & Mohammadpour, H. (2016). Isolation and Molecular Characterization of Non-Sorbitol Fermenting Escherichia coli Isolated From Fresh Ground Beef. Jundishapur Journal of Health Sciences, 8(1), 48–53. https://doi.org/10.17795/jjhs-31195
MLA
Maktabi, S., Zarei, M., & Mohammadpour, H. "Isolation and Molecular Characterization of Non-Sorbitol Fermenting Escherichia coli Isolated From Fresh Ground Beef", Jundishapur Journal of Health Sciences, 8, 1, 2016, 48–53. doi: 10.17795/jjhs-31195
HARVARD
Maktabi, S., Zarei, M., & Mohammadpour, H. (2016). 'Isolation and Molecular Characterization of Non-Sorbitol Fermenting Escherichia coli Isolated From Fresh Ground Beef', Jundishapur Journal of Health Sciences, 8(1), pp. 48–53. doi: 10.17795/jjhs-31195
CHICAGO
Maktabi, S., Zarei, M. & Mohammadpour, H., "Isolation and Molecular Characterization of Non-Sorbitol Fermenting Escherichia coli Isolated From Fresh Ground Beef." Jundishapur Journal of Health Sciences, 8 1 (2016): 48–53, doi: 10.17795/jjhs-31195
VANCOUVER
Maktabi S., Zarei M., Mohammadpour H. Isolation and Molecular Characterization of Non-Sorbitol Fermenting Escherichia coli Isolated From Fresh Ground Beef. Jundishapur J Health Sci. 2016;8(1):48–53. doi: 10.17795/jjhs-31195
